Imodoka ya cab electrophoresis itanga umusaruro

1. Igikoresho cya electrophoreque yubuso bwicyuma rusange, inzira yacyo ni: kubanza gukora isuku → kumurongo → gutesha agaciro → gukaraba → gukuramo ingese → gukaraba → kutabogama → gukaraba → fosifatiya Kuma → kumurongo.

2. Substrate hamwe no kwiyitirira igifuniko bigira uruhare runini kuri firime ya electrophoreque.Ubusanzwe abaterankunga bakoresha umusenyi cyangwa kurasa kugirango bakureho ingese, hamwe nudodo twa pamba kugirango bakureho umukungugu ureremba hejuru yumurimo wakazi, hamwe na 80 # ~ 120 # impapuro zumucanga kugirango bakureho ibyuma bisigaye hamwe nizindi zuba hejuru.Ubuso bwibyuma bivurwa no gukuramo amavuta no gukuramo ingese.Iyo ibisabwa hejuru biri hejuru cyane, fosifatiya na passiyo yo kuvura birashobora gukorwa.Igikoresho cyicyuma kigomba kuba fosifati mbere ya anodic electrophoreis, bitabaye ibyo kurwanya ruswa ya firime irangi.Kuvura fosifatiya, mubisanzwe hitamo zinc umunyu wa fosifatique, uburebure bwa 1 ~ 2 mm, bisaba korohereza neza ya firime ya fosifati.

3. Muri sisitemu yo kuyungurura, muri rusange gukoresha akayunguruzo, kuyungurura imiterere yimifuka ya mesh, aperture ya 25 ~ 75μm.Irangi rya electrophoreque ryungururwa binyuze mumashanyarazi ahagaritse gushungura.Urebye ibintu nkigihe cyo gusimbuza nubwiza bwa firime, akayunguruzo hamwe na aperture 50μm nibyiza.Ntishobora gusa kuba yujuje ibisabwa bya firime, ariko kandi ikemura ikibazo cyo guhagarika umufuka.

4. Ubwinshi bwikwirakwizwa rya sisitemu ya electrophoreque bigira ingaruka itaziguye kumazi yo koga hamwe nubwiza bwa firime.Hamwe no kwiyongera kwizunguruka, imvura nububwa muri tank bigabanuka.Nyamara, gusaza kwa tank birihuta, gukoresha ingufu biriyongera, kandi ituze ryikigega rirakomera.Nibyiza kugenzura umubare wikwirakwizwa ryamazi ya tank inshuro 6 ~ 8 / h, ntabwo ari ukwemeza gusa ubwiza bwa firime, ariko kandi no gukora neza.

5.Hamwe nigihe kinini cyo gukora, impedance ya anode diaphragm iziyongera, kandi imbaraga zakazi zizagabanuka.Kubwibyo, ukurikije igihombo cya voltage mumusaruro, imbaraga zumuriro wamashanyarazi zigomba kwiyongera buhoro buhoro kugirango zishyure igabanuka rya voltage ya diode ya anode.

6.Sisitemu ya Ultrafiltration igenzura kwibanda kumyanda yanduye yazanwe mukazi kugirango ireme neza.Mu mikorere yiyi sisitemu, hagomba kwitonderwa imikorere idahwitse ya sisitemu nyuma yo gukora, guhagarika ibikorwa birabujijwe rwose kugirango wirinde gukama kwa ultrafiltration.Amababi yumye hamwe na pigment yumira kuri ultrafiltration membrane kandi ntibishobora gusukurwa neza, bizagira ingaruka zikomeye mubuzima bwimikorere ya ultrafiltration.Igipimo cyimyanda ya ultrafiltration membrane kigabanuka nigihe cyo gukora, kandi kigomba guhanagurwa rimwe muminsi 30 kugeza 40 kugirango amazi ya ultrafiltration asabwa kumeneka no gukaraba.

7. Uburyo bwa elegitoronike ya elegitoronike ikwiranye numubare munini wumurongo.Inzira yo gusimbuza ikigega cya electrophoreis igomba kuba munsi y'amezi 3.Dufashe umurongo wa electrophoreis hamwe numusaruro wumwaka wa 300.000 impeta yicyuma nkurugero, ni ngombwa cyane gucunga neza siyanse ya tank.Ibipimo bitandukanye byamazi yamazi bipimwa buri gihe, kandi amazi ya tank arahindurwa agasimburwa ukurikije ibisubizo byikizamini.Mubisanzwe, ibipimo byamazi ya tanki bipimwa kumurongo ukurikira: agaciro ka PH, ibirimo bikomeye hamwe nubushobozi bwumuti wa electrophoreis, ultrafiltration hamwe na ultrafiltration isuku yumuti, cathode (anode), kuzenguruka kumesa hamwe nigisubizo cyogukora isuku rimwe kumunsi;Ikigereranyo cyibanze, ibinyabuzima bikungahaye, laboratoire ikizamini cya kabiri kabiri mu cyumweru.

8. Ubwiza bwimicungire ya firime, bugomba kenshi kugenzura uburinganire nubunini bwa firime, isura ntigomba kugira pinhole, itemba, igishishwa cya orange, iminkanyari nibindi bintu, kugenzura buri gihe guhuza firime, kurwanya ruswa nibindi byumubiri kandi ibipimo by'imiti.Kugenzura ukwezi ukurikije ibipimo byubugenzuzi, muri rusange buri kintu kigomba kugeragezwa.

Gukoresha amashanyarazi ya electrophoreque hamwe n irangi ryamazi byerekana iterambere ryinshi mubikorwa byo gutwikira.

Umuvuduko wubwubatsi bwa electrophoreque, gukoresha imashini no kwikora birashobora kugerwaho, gukora ubudahwema, kugabanya ubukana bwumurimo, firime imwe yo gusiga amarangi, gukomera cyane, kuburyo rusange bwo gutwikira ntabwo byoroshye gutwikirwa cyangwa gutwikirwa nabi, nkimbavu zavuzwe haruguru, gusudira n'ahandi hantu hashobora kubona ndetse, firime nziza.Igipimo cyo gukoresha amarangi kugera kuri 90% -95%, kuko irangi rya electrophoreque ni water nkigisubizo, kitari - cyaka, kitari uburozi, cyoroshye gukora nibindi byiza.Amashanyarazi yumye ya electrophoreque, hamwe na adhesion nziza, irwanya ingese, irwanya ruswa, irwanya ikirere nibindi bintu biruta irangi risanzwe hamwe nuburyo rusange bwo kubaka.

Byakoreshejwe muburyo bwose bwo gushushanya, izindi moderi zirashobora gutegurwa.